Are there any differences between using serum or plasma samples?
In some cases there will be a difference in the dilution needed to eliminate any matrix effect, but frequently they respond in a similar fashion. Refer to the kit insert instructions for the specific details since the response can vary from kit to kit. Q: I would like to run liver homogenates in one of your CorrelateTM EIA kits but am afraid that the endogenous alkaline phosphatase levels will interfere in the assay. A: Alkaline phosphatase in the liver should not contribute to the assay as it will not be bound and therefore lost during the wash step. As a precaution, it would be advisable to ensure that the plate is washed thoroughly.
