Are there any changes required in the retrieval technique fpr Immunohistochemsitry (IHC)?
There will be reduced need for an aggressive and lengthy retrieval process. Why? If using microwave fixation and histoprocessing, you have reduced exposure times to the adverse effects of antigen masking/preservation associated with reagents in conventional histoprocessing. If tissues are formalin fixed with longer conventional times, followed by microwave processing, there will be little or no change to your existing retrieval process and antibody dilutions.
