Are there antibodies available for western blot detection?
50 µl of rabbit serum (#S6654S) raised against the Bacillus circulans chitin binding domain (CBD) is provided with the IMPACT kit. A 1:5000 dilution of anti-chitin binding domain serum (Polyclonal anti-CBD) may be used to analyze crude extracts or purified samples. A western blot is carried out when the fusion protein is not detectable by Coomassie staining. NEB also sells murine anti-CBD monoclonal antibody (#E8034S), which can be used at 1:1000. The advantage of the monoclonal antibody is that it has higher specificity and results in lower background compared to the polyclonal antibody. Rabbit serum raised against the S. cerevisiae VMA1 intein is available upon special request.
