Are the prenatal probes designed for use on paraffin embedded tissue, abortive tissue or placenta? If so which protocol should be used?
Our Prenatal range is developed for use with uncultured amniocytes so product performance with other cell types cannot be guaranteed. However if the intention is to use prenatal probes on such tissue sections, a solid tumour pre-treatment protocol for paraffin embedded tissue sections could be used, including removal of paraffin wax (de-paraffination), rehydration, acid treatment, pre-treatment and protein digestion. For cells fixed in Carnoy’s fixative, the Prenatal pre-treatment protocol should be followed. In all cases where the prenatal probes are used for samples not recommended by Cytocell, the recommended protocol should be followed first to establish expected probe brightness and hybridisation efficiency before deviations from the protocol are considered. Cytocell can only guarantee results on recommended tissue types, however.
