Are the insulin syringes with the needle already attached ok for the injections?
As far as the injection process is concerned, it is probably ok. However, those syringes have tick marks at 10 ul that are extremely close together, and your ability to inject anywhere near a reproducible 5 ul volume is unlikely. 50 ul glass Hamilton syringes with a luer tip have tick marks at every microliter; therefore, you can inject almost exactly 5 ul with reproducibility. (This is independent, however, of the rather unpredictable back-flow when you remove the needle that is common to any injection techique. The myocardium is, after all, a solid tissue that is squeezing itself.
As far as the injection process is concerned, it is probably ok. However, those syringes have tick marks at 10 ul that are extremely close together, and your ability to inject anywhere near a reproducible 5 ul volume is unlikely. 50 ul glass Hamilton syringes with a luer tip have tick marks at every microliter; therefore, you can inject almost exactly 5 ul with reproducibility. (This is independent, however, of the rather unpredictable back-flow when you remove the needle that is common to any injection techique.