Are the amount of DNA/per reaction enough to proceed with downstream applications ?
For arrays 250 ng at a minimum are needed and 1-2 ug per array the most likely. For ChIP, there are many factors influencing the recovery of DNA. The final amount recovered depends on cell type, number of starting cells and the antibody. DNA must be pooled or amplified following the next methods: Whole Genome Amplification (WGA), Ligation Mediated-Polymerase Chain Reaction (LM-PCR), or T7 linear Amplification (Method of choice – Diagenode is working actually in a kit for T7 linear amplification). For MeDIP you should pool up MeDIP samples as well. (~50-100 ng/sample using manual kit) For sequencing 5-10 ng/per ChIP are needed Auto ChIP experiment with Diagenode histones antibodies and chromatin from 10^5 cells provide in general more than 10 ng of DNA per ChIP reaction.
