Are techinques with sufficient throughput available?
Y2H and affinity purification/mass spectrometry both require access to clones. Different collections of clones are available. The latest version of the human ORFeome contains over 12 000 full length clones of human genes 27. Invitrogen has over 35 000 full length clones in their collection while the FLJ-DB database (www.nedo.go.jp/bioiryo/bio-e/index.html) reports over 20 000 clones (although not necessarily full length). Although the availability of full length clones has improved drastically, the annotation and quality of clones remains an important issue. It is likely that clones representing most human genes will be readily available over the next few years.
