Are specimen and virus storage containers leak-proof with externally threaded screw caps?
Failure to use containers of this design can lead to adverse events when containers are opened e.g. creation of aerosols or splashes, contamination of the outside of containers and contamination of the hands of workers. Are specimens and inoculated cell cultures only opened and manipulated in a class II biosafety cabinet designated for infectious work? Observations should also include cabinet airflow and work practices within the cabinet, particularly the presence of materials and equipment that may impede airflow. Also important are verification and certification of maintenance checks of BSCs, and use of appropriate disinfectants for their decontamination. Are media mixed or poured from inoculated cultures or are there other avoidable opportunities for creating aerosols? The creation of aerosols or splashes can result in contamination of the worker, equipment, the environment or the work. Numerous opportunities exist in virology laboratories for creation of aerosols or splashes, e.g.
