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Are SNAP-tag substrates stable to fixation?

April 26, 2017fixation snap-tag STABLE substrates

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Are SNAP-tag substrates stable to fixation?

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The fluorescence from SNAP-tag substrates is derived from an organic fluorophore. Therefore labeled SNAP-tag fusion proteins are very stable to fixations and do not lose signal intensity in contrast to some GFP spectral variants.

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Yes. SNAP-tag substrates are derived from organic fluorophores which are stable to fixation. Fluorescently-labeled SNAP-tag fusion proteins do not lose signal intensity in contrast to some GFP spectral variants. After labeling the SNAP-tag fusion proteins, the cells can be fixed with standard fixation methods such as para-formaldehyde, ethanol, methanol, methanol/acetone etc. without loss of signal.