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Are samples containing EDTA or reducing agents compatible with the kit?

April 26, 2017agents compatible containing EDTA kit reducing samples

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Are samples containing EDTA or reducing agents compatible with the kit?

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No. Chelating agents such as EDTA and EGTA will cause metal to leach from the column and hence, reduce binding. Reducing agents such as DTT, 2-mercaptoethanol and TCEP also reduce binding to the column. Remove incompatible substances from the sample by desalting before applying to the column.