Are impurities in my kinase or protein substrate preparation detrimental to the Transcreener™ ADP Assay?
The Transcreener™ADP Assay measures the production of ADP from whatever source. Therefore, any impurity that causes ADP production such as a contaminating kinase, phosphatase or ATPase will interfere with accurate measurement of the desired kinase activity. Care should be taken to minimize these potential contaminants in both kinase and protein substrate preparations.
