Are immuno-electron microscopy services available?
Yes, we will perform immuno-electron microscopy on your sample. In addition to providing the sample, you must provide the primary antibody for your study. Just as for any antibody study, the quality (specificity, affinity, and purity) of your antibody is critical for success. For immuno-electron microscopy, we caution you that Western blots are not a sufficient test of antibody quality. Westerns typically immobilize denatured peptides as antigens, whereas fixed or live cells and tissues have natively folded proteins (and protein complexes). Not only do antibodies lose apparent specificity when probing native rather than denatured peptides, protein complexes and cellular structures may prevent accessibility to antigenic epitopes. Please see the FAQ for immuno-electron microscopy for a more thorough discussion of the pre-requisites for successful immuno-electron microscopy.
