Are FBM media compatible with colored versions of PCR polymerases?
PCR products produced using colored polymerases have been analyzed on FBM gels. No difficulties are reported by our customers to date. With these products, a separate loading medium usually is not added to the PCR reaction prior to loading on a gel. Note, however, that the higher salt content of an undiluted PCR reaction will not produce the highest resolution band separation when compared to a lower-salt sample.
