Are Centriole Organizers Segregated to Acentriolar Daughter Cells?
In mammalian cell lines, the generation of a new centriole is believed to arise from a structure associated with the preexisting centriole. As suggested earlier (Mazia et al., 1960; Sluder and Rieder, 1985a; Sluder et al., 1989), a nontubulin structure could be responsible for the assembly of a new centriole. This conservative mechanism implies that the constitutive loss of the centriolar structure (Debec et al., 1982) or the microsurgical removal of the whole centrosome (Maniotis and Schliwa, 1991) would lead to the inability of the cell to generate a new centriole. In the present work, we observed the regeneration of centrioles after their complete disappearance, suggesting either that centriole organizers were preserved in transiently acentriolar cells, or that de novo centriole formation occurs in this system. We were not able to identify a structure that could correspond to such centriole organizers, but the fact that GT335 antibody acts through the destabilization of microtubules
