Are any changes required from standard DNA synthesis and deprotection?
RESPONSE:These supports (20-3000, 20-3100, etc.) are designed for oligodeoxynucleotide synthesis when a ribose functionality is desired at the 3′-terminus. Following periodate oxidation of the 1,2-diol, labelling or carrier molecules containing amino groups can be conjugated to the 3′-terminus. An example (1) is the use of poly-lysine as a carrier molecule. Details of these procedures are given(2) in the Glen Research Guide to Modification and Labelling. These supports can be used for RNA synthesis, and the conventional RNA supports (20-3300, 20-3400, etc.) (now containing 2′-acetyl protecting groups, which are completely base-labile) can also be used for DNA modification. REFERENCE(S): (1a) M. Lemaitre, B. Bayard, and B. Lebleu, Proc. Natl. Acad. Sci. USA, 1987, 84, 648-652. (1b) M. Lemaitre, C. Bisbal, B. Bayard, and B. Lebleu, Nucleosides & Nucleotides, 1987, 6, 311-315. (2) User Guide to DNA Modification and Labelling, 1999, 70-71.
