Are All Nucleotide Positions in the Inverted Repeats Important?
We were surprised at the degree to which transposition was suppressed between Himar1 and Mpmar1, given that their ITRs are 83% identical. Such a degree of difference is often seen between the two ITRs in the same transposon in other systems. For example, the bacterial insertion sequence IS50 has inverted terminal repeats (the “outer end” and “inner end”) that differ at 7 out of 19 bp (i.e., 63% identical) (Sasakawa, Carle, and Berg 1983 ). Indeed, even Mos1 mariner has mismatches between its ITRs which make each end ca. 90% identical to the other (Maruyama, Schoor, and Hartl 1991 ). One way to analyze homologous sequences to determine important conserved positions is through sequence logo analysis (Schneider and Stephens 1990 ). We generated a sequence logo of the ITRs used in this study and two other mariners from two other subfamilies (C.elegans.mar1 and B.mori.mar1; see fig. 1 ), which are representative of mariner element diversity as a whole (fig. 4 ). The sequence logo strongly s
Related Questions
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- Are All Nucleotide Positions in the Inverted Repeats Important?
