Antibody addition Q. How much primary and secondary antibody should I add?
• If you know the concentration used in standard immunodetection, use 3- to 5-fold higher concentration in 1/3 of the volume (3 mL for single well blot holder). Further optimization will likely be required. See application note entitled: “Triple Well Blot Holder as a Tool for 30-Minute Western Blotting Optimization with the SNAP i.d. Protein Detection System” (AN1965EN00).
