After selection with puromycin, why are there no surviving cells?
The cells you are working with may be sensitive to puromycin. The concentration of puromycin added to the cells may be too high. For each new cell type used, it is recommended that a puromycin titration be performed to determine the lowest concentration of puromycin needed to efficiently select transfected or transduced cells. Also, be sure to wait 48 hours post-transduction before applying antibiotics to the cells. Alternatively, a higher MOI or transduction enhancement methods, such as ExpressMag® may be required for an effective transduction.
