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After MeDIP and column purification, I got less than 20 ng of DNA. What is an expected yield after column purification, 14 ng – 33 ng per IP?

April 26, 2017column dna GOT medip Ng purification yield

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After MeDIP and column purification, I got less than 20 ng of DNA. What is an expected yield after column purification, 14 ng – 33 ng per IP?

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The recovery after MeDIP can vary for different cell type but an average recovery is between 20-50 ng per IP when DNA is purified by phenol-chloroform extraction. Columns-based purification results in some DNA lost comparing to phenol-chloroform extraction. The values you obtained seem to be correct.