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After doing a MERmaid prep from agarose, my eluate solidified. Why?

April 26, 2017agarose doing eluate Mermaid prep solidified

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After doing a MERmaid prep from agarose, my eluate solidified. Why?

1 Answer

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10 Posted

A. Sometimes the agarose is not sufficiently melted in step 1 and can carry over into the final product. To avoid agarose carryover, do the optional wash with the High Salt Binding Solution in step 3b in the MERmaid protocol or wash with High Salt Wash in step 2 of the MERmaid Spin protocol.