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added onto a protein after labelling with each CyDye™ DIGE Fluor minimal dye?

April 26, 2017added cydye™ dige dye fluor labelling protein

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added onto a protein after labelling with each CyDye™ DIGE Fluor minimal dye?

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The molecular weight of each CyDye DIGE Fluor is provided on the specification sheet supplied with each dye (TFA salt). The molecular weight added after labelling can be calculated from this by using the formulae below. Note: The calculation for proteins labelled using CyDye DIGE Fluor minimal dyes is different from the calculation for proteins labelled with CyDye DIGE Fluor saturation dyes. Example of a calculation for minimal dyes (CyDye DIGE Fluor Cy™2 minimal dye); Quoted mass of dye (TFA salt) = 663.62 Mass of dye cation (-TFA) = 663.62 – 113.02 = 550.60 Mass added to protein (-NHS, -H from protein amine group) = 550.60 – 114.08 – 1 = 435.52 The masses added for each of the other two minimal dyes can be calculated in a similar manner; CyDye DIGE Fluor Cy3 minimal dye, mass added to protein = 467.69 CyDye DIGE Fluor Cy5 minimal dye, mass added to protein = 465.