how sensitive are microarrays?
Affymetrix claims that on a routine basis they can detect 1 molecule in 100,000 and that they can detect two fold changes in RNA expression levels. In an optimal hybridization they claim to detect one molecule in 2,000,000 and to detect 10% changes in RNA expression levels. I haven’t seen any published results on spotted arrays but I’d love to hear about it if someone else has.
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- how sensitive are microarrays?