How is the ALT test run on Cholestech LDX Analyzer?
• Fingerstick samples must be applied immediately after collection. Do not let the blood sample sit in the capillary before dispensing into the sample well. • When running controls, serum, whole blood, proficiency or calibration verification material, use the tan colored 35L Mini pipette for ALT. • There is a separate Cholestech control available for use with the ALT test cassette. Do not use the lipid + glucose control for ALT. • Hematocrit levels between 30% and 50% do not affect results. Question: Are there any other differences between the ALT test and the Lipid test? Answer: Assays for ALT may be run at different temperatures, 30C or 37C. The Cholestech LDX ALT assay is run at 37C. Enzyme levels are very dependent on temperature, with higher temperatures producing higher ALT results. If you are going to compare the LDX results with another method make sure the comparison method is run at 37C. ALT methods run at 30C will show lower results. Question: Why do I have to run the Optics
