What is GPC???????????????
Size exclusion chromatography (SEC) is a method in which polymers are separated based on their molecular sizes or strictly speaking hydrodynamic radii. Gel permeation chromatography (GPC) is a term that specifically applies to SEC when an organic solvent is used as the mobile phase. GPC is a procedure used in polymer chemistry for measuring the distribution of molecular mass. Common to all GPC studies is that a polymer sample, which by definition consists of a distribution of (mainly) linear molecules that differ in the number of repeating chemical units, is delivered to a column that is packed with porous particles. Molecules go through the column at rates which are dependant upon their size. Larger molecules cannot enter the pores, therefore they pass quickly through the column and elute first. Smaller polymers enter the pores and take longer to exit the column. Various detectors are used to sense when the polymers elute from the column; the output of the detector is a molecular size
