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How is antibody titer determined?

April 26, 2017Antibody titer

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How is antibody titer determined?

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We test the serum from the immunized mice by ELISA. A fixed amount of antigen is coated on the microtiter plate. Serial dilutions of the serum from 1:1000 to 1:100,000 are prepared to determine the detectable titer of Ab in the serum specific to the target antigen.