Why Use Digital Deconvolution?
Unlike the confocal configuration described above, digital deconvolution microscopy uses the entire fluorescence signal collected by the objective lens without using pinhole to deliver the emitted light to 2D high-sensitivity CCD cameras. The “out-of-focus” flare introduced into the imaging at different optical sections with its subsequent image degradation is reversed by computer deconvolution through the use of a pointspread function (PSF) of the imaging system! By modeling the microscope optics as a linear and shift-invariant system, the PSF can be used to describe the transformation of any image by the microscope. A typical fluorescence microscope image can be: [measured image] = [PSF] * [desired image] where ‘*’ symbol represents the mathematical operation of convolution. The deconvolution (*-1), the mathematical inverse of convolution can be represented as: [desired image] = [measured image] (*-1) [PSF] The goal of deconvolution is to solve the equation for the desired image. Dig
