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What is an ELISpot?

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What is an ELISpot?

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ELISpot is an abbreviation for Enzyme Linked Immunospot Assay. A capture antibody specific for the protein of interest is coated onto a membrane. Cells are added. After an incubation period, the protein secreted from the cells is detected using a biotinylated antibody specific for a different epitope of the protein. Using a SA-AP conjugate followed by the chromogen BCIP/NBT, a colorimetric image of the antigen secreting cells is revealed. The number of antigen secreting cells can be quantified using an automated ELISpot plate reader or manually using a stereomicroscope.

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