What is MPSS?
MPSS stands for Massively Parallel Signature Sequencing, a technique invented and commercialized by Solexa, Inc. of Hayward, California. MPSS and related technologies have been described in publications by Brenner et al. (Nature Biotechnol. [2000] 18:630-634, and PNAS [2000] 97:1665-1670). Like SAGE (Serial Analysis of Gene Expression), MPSS identifies short sequence signatures produced from a defined position within an mRNA, and the relative abundance of these signatures in a given library represents a quantitative estimate of expression of that gene.
Related Questions
- Can every gene be identified in the MPSS analysis? What are the technical biases that may affect the analysis of a particular gene?
- Is MPSS like microarray expression analysis systems, with a hybridization-based query of an mRNA population?
- How frequent are sequencing errors in MPSS and how are they handled?
