How does MVS work?
MVS employs a dual-dye, dual-wavelength, ratiometric absorbance-based measurement method for calculating the volume of sample dispensed into a well of a microtiter plate. The results of the verification tests are traceable to national and international standards. Specially formulated Sample Solutions containing dyes of known concentration are dispensed into dimensionally-characterized microtiter plate wells. The plate is mixed on a microtiter plate shaker to ensure homogeneity of samples. Absorbance readings are taken in the plate reader at 520 nm and 730 nm. The system software, MVS Data Manager, creates a summary report containing the calculated dispensed volume for each dispensing tip. Accuracy and precision of the dispensed volumes are measured against user-defined tolerance limits and failing values are flagged in the report. Summary statistics by well, by row, and by column are also contained in the summary report. Results are saved in a secured database using the MVS Data Manage