Why use cryo-SEM?
Many materials are sensitive to the vacuum conditions and/or the high electron beam energy in the SEM. These include biological and other “hydrated” materials, also low melting point or volatile specimens, even liquids. In addition materials which are normally soft at room temperature can be fractured under cryogenic conditions to expose internal microstructure and the dispersion of components and phases in a system such as an emulsion or suspension. A process or (setting) can be observed as a time resolved series of frozen samples.
