What is Cycle Sequencing?
Cycle sequencing is a simple method in which successive rounds of denaturation, annealing, and extension in a thermal cycler result in linear amplification of extension products (see Figure 3). The products are then loaded onto a gel or injected into a capillary. All current ABI PRISM® DNA sequencing kits use cycle sequencing protocols.
Cycle sequencing is a technique that uses a thermal-cycling procedure similar to PCR amplification for obtaining nucleotide sequence information from DNA samples. The advantages of cycle sequencing are that it is unnecessary to clone a particular gene in order to get it’s DNA sequence and that it requires very little starting DNA material. The method is very similar to the standard dideoxy sequencing , but uses the elements of PCR for amplifying the terminated oligonucliotides used in the sequencing. For more information see Rao.Saunders1992, Ruano.Kidd1991 and Smith1990.
