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How much DNA is required?

dna
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How much DNA is required?

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Starting DNA= 100 nanograms per 100 µl PCR reaction is recommended for the LR PCR protocol. DNA amplicon/Array= 250 pM per array or ~ 50 f moles/PCR reaction, we recommend that the user be able to amplify at least ~100 femtoMoles per individual fragment or PCR amplicon.

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