What is TA Cloning?
A method of cloning PCR products without the use of restriction enzymes, exploiting the terminal transferase activity of some DNA polymerases. PCR products are amplified with Taq polymerases lacking 5´->3´proofreading activity, which add a 3´-A overhang to each end of the PCR product. Such PCR products will be ligated to a linearized vector with complementary 3´-T overhangs using T4 DNA ligase. This method does not allow for a directional cloning of the insert sequence.
