How Do You Make A Tris Buffer?
Tris is a chemical with basic properties, having a pKa of 8.1. It can be used to buffer solutions from drastic pH changes, keeping them in the pH range of 7.0 to 9.0. Make any Tris-HCl buffer in this pH range, at any molarity using these simple stepsDifficulty: EasyTime Required: 10 minutesHere’s How: • Calculate Moles of Tris Base Determine the number of moles of Tris base needed by multiplying the desired molar concentration of buffer by the volume of buffer being made. mol/L * L = moles needed • Calculate Mass of Tris Base Determine the mass of Tris base to weigh by multiplying the number of moles by the molecular weight (121.14 g/mol) of Tris. moles needed * g/mol = g • Dissolve Tris Base in Water Dissolve the required mass of Tris into a volume of deionized water approximately 1/3 of the desired volume of buffer to be made. • Adjust the pH Using a pH meter, titrate the solution of Tris with 1M hydrochloric acid (HCl) until the correct pH is reached. • Bring to Volume Add the TrisH
