What is DNA Cloning?
DNA cloning is also known as molecular cloning, recombinant DNA technology, and gene cloning. Accordingly, the biological materials being cloned in DNA cloning are the DNAs. Therefore, DNA cloning is the simplest type of cloning. Scientists use recombinant DNA technology to produce proteins (protein expression & purification), cell transfection for studying protein function in certain cells, and for other biological applications. Ethical aspect of DNA cloning Since the process of DNA cloning itself does not cause any harm in living creatures, DNA cloning certainly does not evoke any ethical issues in Buddhism. DNA cloning is a common biological technique widely and freely used in many science laboratories. 2. Therapeutic cloning What is therapeutic cloning? Therapeutic cloning is the process of cloning tissues or organs, in which the cloned tissues/organs would only be used for therapeutic purposes. The process of therapeutic cloning involves a procedure called somatic cell nuclear tra
DNA cloning – also known as molecular cloning, gene cloning and recombinant DNA technology – refers to the process of creating multiple copies of an isolated DNA fragment or fragments by in vitro or in vivo methods. It is possible to clone entire gene fragments, random portions of DNA fragments or specific DNA sequences. Apart from DNA cloning, two other main cloning types are reproductive cloning, which is concerned with human and animal cloning, and therapeutic cloning, concerned with embryonic cloning to harvest stem cells for research and potential medical treatment purposes. There are various procedures for DNA cloning, but some steps are constant for all. The process of DNA cloning begins with isolating a DNA fragment or fragments of interest from the chromosomal DNA using restriction enzymes or chemically synthesized oligonucleotides. Other methods for accomplishing this include different procedures like polymerase chain reaction (PCR), agarose gel electrophoresis and DNA sonica
