What is Annexin V?
· 35 KDa, Ca++ dependent, protein · Present in many tissues (endothelial cells, placenta, …) · Present in platelets (5.9 10 g/cell and, at higher concentration, in red blood cells and leukocytes · Binds to anionic phospholipids via Ca++ · Inhibits blood coagulation and phopholipase A · Regulates exocytosis and interacts with cytoskeletal proteins · Inhibits pro-coagulant and pro-inflammatory activities of dying cells during apoptosis Diagnostic Value of Annexin V Assay · Autoimmune disease and Systemic Lupus Erythematosus (SLE) (10 to > 250 ng/ml) · Early stage of myocardial infarction (10 to > 50 ng/ml) · Hepatic disorders (10 to > 100 ng/ml) · Follow-up of patients treated with Annexin V · Normal range in human plasma: < 10 ng/ml Selected References Released Annexin V during platelet storage correlates with EDTA induced shape change. (P. Krailadsiri et Al.: Transf. Scien. 1997) Annexin V increases from 16.0 ng/ml (± 13.31) to 33.68 ng/ml (± 20.22) during a 5-6 day platelet concentr
