what is shifting?
Shifting means that if peptide # 1 contains sequence aa 1-12 of a protein then if we shift by 2 amino acids the second peptide will have sequence aa 3-14. This is to ensure that we isolate the exact amino acid sequence that the probe is binding to. We find that shifting by 2or 3 is ideal. Ex. HRFACGQPMNSI FACGQPMNSICG CGQPMNSICGLW If three consecutive spots are positive for example for the above three peptides, then the epitope is assumed to be CGQPMNSI which is common.
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