Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

only with ExpressArt?

April 26, 2017expressart

0

10 Posted

only with ExpressArt?

1 Answer

0

10 Posted

All other kits use random priming with high amplification yields for rRNAs which leads to reduced sensitivities: lower number of detected genes. This can be avoided by an extra step for (partial: ~70%) removal of rRNAs. This step uses magnetic beads which require an absolute minimum of 1 μg of total RNA, otherwise all RNA will be lost during the procedure. All other kits offer only one amplification round. ExpressArt avoids random priming: the patented TRinucleotide priming selects against rRNAs (~98% removal) and ExpressArt offers two (even three) amplification rounds.