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What is mtDNA sequencing?

mtDNA sequencing
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What is mtDNA sequencing?

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Sequencing involves reading the letters of the DNA. In total we read 1,144 bases covering the Hyper Variable Regions of HVR1, HVR2 and HVR3.

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The common abbreviation for mitochondria is mtDNA. Mitochondiral molecules are present in 100s-1000s of copies per cell, as opposed to the nuclear DNA, which is present in just two copies per cell. The abundance of mtDNA allows discrimination among individuals or biological samples, particularly if nuclear DNA is degraded or unavailable.

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