How do the MycoSep® columns work?
The MycoSep® is a simple 10 to 30-second clean up format. The MycoSep® clean up column is pushed into a test tube (containing the extract), forcing the extract to filter upwards through the chemical packing in the column. The interferences adhere to the chemical packing in the column and the purified extract passes through a membrane (frit) to the surface of the column. Nevertheless, it should be taken into consideration that the interferences and the analyte is separated chromatographically. The consequence is that the interferences are not bound to the column unlimited. If too much extract is pushed through the column interferences might be again eluted leading to less resolved chromatograms. The optimum volume for a special commodity might be slightly different from the volume we suggest for most common commodities.
