What is dns reagent?
DNS is DiNitroSalicylic acid. The reagent is used for determining sugar content, but especially Glucose. A typical method to make it would be: Slowly add 10.6 grams of 3,5 – Dinitrosalicylic acid and 19.8 grams of Sodium hydroxide to 1.416 liters of distilled or deionized water. Mix until all of the solid has dissolved. Add 306 grams of Sodium Potassium tartrate (Rochelle salts), 7.6 mL of melted Phenol and 8.3 grams of Sodium Meta Bisulfite to the solution, Once again mix until all solid has dissolved. For a calorimetric determination of glucose (or reducing sugars), 1.0 to 2.0 mL of sample is placed in a test tube and 3.0 mL of DNS reagent is added. The tubes are place in a boiling water bath for 5 minutes to allow color to develop. The specimen are read in a colorimeter at 550 nm using a water blank.
