Can I use RABIT for biofilm evaluation?
RABIT APPLICATIONS – BIOFILMS There are a number of publications where impedance has been used as a tool in the study of biofilms. Published papers refer to the use of RABIT and other competitor instruments specifically Malthus and Bactometer. Holah et al (1990) described the use of direct impedance to enumerate microbes on steel discs following exposure to 12 disinfectants. The ‘pass’ criterion was a 5 log reduction in test organism viability after 5 min exposure. Similarly, Druggan et al (1993) used indirect impedance with 1 cm diameter steel discs which were directly transferred to the impedance tubes. This avoided the inaccuracy of physical removal of the microbial growth using sand agitation. Sodium hypochlorite was used as the model for chlorine-based disinfectants. The European suspension test organisms Ps.aeruginosa NCIB 10421, Proteus mirabilis NCIB 12596, Staph.aureus NCTC 10788 and S.cerevisiae ATCC 9763 were used to test the efficacy of sodium hypochlorite to microbial biof
