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Can I use colonies from an old plate to amplify DNA or glycerol stocks?

April 26, 2017amplify colonies dna glycerol plate stocks

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Can I use colonies from an old plate to amplify DNA or glycerol stocks?

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Yes. DNA from colonies on plates up to 14 days old (stored at 4 C) have successfully been amplified using TempliPhi kit. However, fresh colonies are recommended whenever possible. For glycerol stocks, dilute 1 l of glycerol stock in 50 l of TE or water ad use 0.5-1 l of diluted stock per amplification reaction.