What determines the retention or hydrophobicity of a molecule in HPLC?
Much of the retention of a column in Reverse Phase chromatography is due to what is commonly called hydrophobicity. This natural phenomena is due mostly to the size of the hydrophobic (water resistant) area of a molecule. Retention will increase with the amount of water in the mobile phase. In general, the more hydrophobic the molecule (see Octanol/Water Partition Coefficient) the longer it should be retained.
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