What is Aqueous Normal Phase?
[top] Normal-Phase Chromatography is defined as a chromatographic phase that will increase the retention of a target compound as the mobile phase becomes less polar (a decrease in concentration of the most polar solvent, often water) conversely the compound has longest retention in a non-polar solvent such as 100% hexane. Reverse-Phase Chromatography is the opposite or the reverse; there is an increase in retention time of the target compounds as the mobile phase becomes more polar (increase concentration of the most polar solvent, often water). Aqueous Normal-Phase Chromatography will be defined as a normal phase separation pattern using the reverse phase solvents Water and Acetonitile. In Aqueous-Normal Phase, the maximum retention time of target compounds is 100% acetonitrile (least polar solvent) and as you increase the polar solvent content (Aqueous), the retention reduces to a minimum when the mobile phase is at 70% Acetonitrile.
