Which is better with TYPE-C Silica based HPLC Columns: TFA, Phosphoric Acid or Formic Acid?
[top] TFA will usually provide better peak shapes than Phosphoric or Formic Acid, but Phosphoric is normally recommended for UV detector use since it is less hazardous than TFA. TFA can cause some detector response – peak suppression with Mass Spec, but most users find this acceptable. If they cannot, then Formic Acid can be used, but we recommend using a combination of Ammonium Formate 20mM with Formic Acid buffered to approximately pH 2.2.
