What is flow cytometry?
Flow cytometry is a technology whereby a stream of cells is passed through a laser beam at high speed (thousands of cells/second). As each cell passes through the laser beam, scattered light is detected by the flow cytometer. If the cell contains molecules that fluoresce at the wavelength of the laser light, fluorescence is emitted; this allows rapid and accurate enumeration of the cells with the property associated with fluorescence of a particular wavelength (for example, labeling with a fluorescent antibody). The raw data are used to identify the different cell populations in the specimen and to infer the characteristics of the cells of interest.
Related Questions
- Are there special considerations I need to take into account when using the eFluorTM Nanocrystal conjugated antibodies for flow cytometry?
- What are the advantages of sorting by Flow Cytometry compared to other isolation techniques (i.e. magnetic bead separation, panning, etc.)?
- What are the policies of the Flow Cytometry Core?
